Cytotoxicity testing is a rapid and cost-effective tool to screen for potential toxicity before a compound enters expensive advance testing and development. MB Research has extensive experience with cytotoxicity in murine keratinocytes and fibroblasts, human and other primary cell lines from different sources.
Cytotoxicity Testing
Test by Direct Contact (MEM Elution)
ISO 10993-5 & ISO 10993-12, USP 23
This assay tests for biocompatibility. The test article is extracted with DMEM media supplemented with 5% fetal bovine serum and tested on monolayer L929 mouse fibroblast cells based on the current ISO 10993‐5 standard. This protocol is a qualitative test to determine the potential of a test article to produce cytotoxicity.
Test by Indirect Contact (Agar Overlay)
ISO 10993-5
This assay tests for cytotoxicity by indirect contact. This assay is not appropriate for leachables that cannot diffuse through the agar or may react with agar. The test article is laid on an agar layer poured above a monolayer of L929 mouse fibroblast cells based on the current ISO 10993-5 standard. This protocol is a qualitative test to determine the potential of a test article to produce cytotoxicity.
Neutral Red Uptake (NRU) Cytotoxicity Test
ISO 10993-5
This assay tests for the cytotoxic potential of a test article when applied to a monolayer of epidermal keratinocytes or fibroblasts in culture.
3T3 Neutral Red Uptake Phototoxicity Test
OECD 432
The assay is designed to detect the phototoxicity induced by the combined action of a test article and light by using an in vitro cytotoxicity assay with the Balb/c 3T3 mouse fibroblast cell line. The test identifies aqueous-soluble compounds (or formulations) that have the potential to exhibit in vivo phototoxicity.
Cytotoxicity – MB Protocol Format
To determine the proliferative or cytotoxic potential of a test article when applied to epidermal keratinocytes or fibroblasts in culture. Can use MTT reduction or Neutral Red Uptake.
