Key Assay Features
- Quick Results
- Based on ECVAM Validated Protocol #442C
- Inexpensive Pre-Screen of New Formulations
- Supports Discrimination between GHS Category 1 and Not Categorized
Direct Peptide Reactivity Assay (DPRA)
The Direct Peptide Reactivity Assay (DPRA) is an in chemico method used to predict epidermal protein binding. Binding of epidermal proteins is the Molecular Initiating Event (MIE) on the sensitization Adverse Outcome Pathway (AOP). The DPRA uses HPLC to measure the depletion of synthetic peptides (Cysteine and Lysine) in solution following exposure to test chemicals.
Regulatory Acceptance - OECD 442C
OECD Test Guideline 442C is accepted for use. DPRA testing is considered validated by EURL-ECVAM, but should be used as part of an intergrated approach to testing and assessment (IATA) for discrimination between sensitizers and non-sensitizers for hazard classification and labelling.
In Vitro Sensitization ITS
MB Research Labs Integrated Testing Strategy
MB Research has developed a non-animal Integrated Testing Strategy (ITS) for chemical-induced contact hypersensitivity (skin sensitization). This ITS provides a completely non-animal alternative to traditional testing methods. Together, our three assays address three key events on the skin sensitization adverse outcome pathway (AOP).
The In Vitro Sensitization Assay (IVSA) is a keratinocyte activation test. IVSA uses an ELISA method to measure IL-18 release from a topically treated reconstructed 3D human keratinocyte tissue model.
The Human Cell Line Activation Test (h-CLAT) is a dendritic cell activation test. The h-CLAT uses flow cytometry to measure CD86 and CD54 expression on treated THP-1 cells in culture.
The Direct Peptide Reactivity Assay (DPRA) is an in chemico method used to predict epidermal protein binding. Binding of epidermal proteins is the molecular initiating event on the AOP. The DPRA uses HPLC to measure the depletion of synthetic peptides in solution following exposure to test chemicals.
Related Skin Sensitization Testing Links
- Murine Local Lymph Node Assay
- Draft Test Guideline - OECD
- ICCVAM Workshop presentation "New Models in the Validation Pipeline for ACD Hazard Testinghuman Cell Line Activation Test: h-CLAT"
- New - In Vitro Sensitization Assay based on IL18 Expression in 3D Human Tissue Constructs
- TOXNote - In Vitro Sensitization Assay
Related In Vitro Toxicology Assays and Links
Genomic Allergen Rapid Detection System
in vitro dermal sensitization based on gene expression analysis. GARDskin, GARDpotency, & GARDair
In Vitro Toxicology Testing
Custom and Standard In Vitro Toxicity Testing Protocols
In Vitro Toxicology Blog by MB Research
Updates and Research Highlights on the development and use of In Vitro test models.
In Vitro Test Models - 3D Tissue Constructs
Toxicity testing using 3D human tissue constructs and other 3D models.
Laboratory Information Links:
Below are MB Research services related to our In Vitro Toxicology Testing services.